대한대장항문학회

Due to increasing incidence of colorectal carcinoma in Korea, this condition becomes a<br>burden to practitioner on their daily work and at same time it became a one of major<br>challenging subject in medical research field in Korea. However survival rate of the<br>disease has not improved since 1950 and there are nothing much offer for this patient<br>others than operation with few exception such chemotherapy.<br>To improve survival rate of colorectal cancer, many researches have been focus on<br>histopathologic feature and molecular biology recently. Histopathologic staging of the<br>rejected tumor is the most important prognostic factor up to now. But it is not enough<br>to be used with fuel confidence in clinical practice. That is why other prognostic factors<br>have been intensively searched and few them are tumor DNA content,953 Protein and<br>epidermal growth factor receptor(EGFR) which are products of molecular biology.<br>But there are no many researches on clinical efficacy of these new Prognostic factors.<br>We carried out this study to see if DNA content, p53 protein and EGFR expression<br>were independent prognostic factors in colorectal carcinoma.<br>To analyse DNA content of cancer, flow cytometry was used. The g53 Protein and<br>EGFR expression were identifiey by immunohistochemical staining using monoclonal and<br>polyclonal antibody. The materials subjected for this study were 56 paraffin-embedded<br>colorectal carcinoma, which were collected from January of 1989 to December of 1990 at<br>department surgery of Korea University Hospital. These Patients, who were able to be<br>followed at least 2 rears after operation, were elected for this study. Of 56 cases,31<br>(55.4%) showed DNA aneuploidy, 27(48.2%) and 30(53.6%) showed positive expression of<br>p53 protein and EGFR.<br>Recurrent and advanced stage tumors were more likely to demonstrate<br>DNA<br>aneuploidy, however these differences did not reach statistical significance. Positive<br>expression of p53 did not correlate any other clinical and pathologic Parameters. Positive<br>expression of EGFR statistically correlate with recurrency in stage B.<br>These data suggest that tumor DNA content and EGFR expressions are important<br>prognostic and adjuvant therapeutic indicator in Patients with stave B colorectal<br>carcinoma Patients.<br>